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【文摘发布】美国学者发现高压氧能治疗动物阳痿

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楼主 sunxjk
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这个帖子发布于11年零317天前，其中的信息可能已发生改变或有所发展。
J Sex Med. 2008 Jan 11 [Epub ahead of print] Links
The Effect of Hyperbaric Oxygen Therapy on Erectile Function Recovery in a Rat Cavernous Nerve Injury Model.Müller A, Tal R, Donohue JF, Akin-Olugbade Y, Kobylarz K, Paduch D, Cutter SC, Mehrara BJ, Scardino PT, Mulhall JP.
Department of Urology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Introduction. Cavernosal oxygenation appears to be important for preservation of erectile tissue health. Hyperbaric oxygen therapy (HBOT) has been shown to improve tissue oxygenation and has neuromodulatory effects. Aim. This study was designed to define the effects of HBOT on erectile function (EF) and cavernosal tissue in the rat cavernous nerve (CN) injury model. Methods. Four groups of Sprague-Dawley rats were studied: rats with bilateral CN crush, HBOT treated (Crush+/HBOT+); bilateral CN-crush/no HBOT (C+/H-); no crush/no HBOT (C-/H-); and no crush/HBOT (C-/H+). HBOT was delivered daily for 90 minutes at three atmospheres for 10 days commencing the day of CN crush. Main Outcome Measures. Ten days after CN injury, the animals underwent CN stimulation measuring the maximal intracavernosal pressure/mean arterial pressure (ICP/MAP) ratios. Corporal tissue was harvested pre-sacrifice, and immunohistochemically stained for nerve growth factor (NGF), endothelial nitric oxide synthase (eNOS), and cluster of differentiation molecule (CD31). Histologic analysis was performed for Masson's trichrome to assess the smooth muscle-collagen ratio. Terminal deoxynucleotidyl transferase Biotin-dUTP Nick End Labeling assay was used to define apoptotic indices (AIs). Results. The C+/H- group had significantly lower ICP/MAP ratios compared with C-/H- rats, (31% vs. 70%, P < 0.001). C+/H+ rats had significantly higher ICP/MAP ratio recovery compared with the C+/H- group (55% vs. 31%, P = 0.005). NGF and eNOS staining densities were higher in C+/H+ rats compared with C+/H- rats (P < 0.05 and P < 0.001, respectively). No difference was seen in CD31 expression. Staining density for MT displayed a trend toward higher smooth muscle preservation after HBOT. AIs were significantly increased by HBOT (P < 0.05). Conclusion. HBOT following a CN injury improved EF preservation in this model, supporting the cavernosal oxygenation concept as protective mechanism for EF. The effects appear to be mediated via preservation of neurotrophic and endothelial factor expression.

PMID: 18194179 [PubMed - as supplied by publisher
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2008-01-26 12:41 浏览 : 1735 回复 : 5
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本人认领本文编译，若48小时未上传译文，请其他战友自由认领
2008-01-26 22:52
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J Sex Med. 2008 Jan 11 [Epub ahead of print] Links
The Effect of Hyperbaric Oxygen Therapy on Erectile Function Recovery in a Rat Cavernous Nerve Injury Model.Müller A, Tal R, Donohue JF, Akin-Olugbade Y, Kobylarz K, Paduch D, Cutter SC, Mehrara BJ, Scardino PT, Mulhall JP.
Department of Urology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
高压氧----海绵体神经损伤的大鼠重新勃起
Introduction. Cavernosal oxygenation appears to be important for preservation of erectile tissue health. Hyperbaric oxygen therapy (HBOT) has been shown to improve tissue oxygenation and has neuromodulatory effects. Aim. This study was designed to define the effects of HBOT on erectile function (EF) and cavernosal tissue in the rat cavernous nerve (CN) injury model.
介绍：海绵体氧疗在勃起组织的健康方面有重要意义。高压氧疗法（HBOT）可提高组织氧合和促进神经调节。通过研究大鼠海绵体神经损伤模型以了解HBOT在治疗勃起功能和海绵体组织方面的作用。
Methods. Four groups of Sprague-Dawley rats were studied: rats with bilateral CN crush, HBOT treated (Crush+/HBOT+); bilateral CN-crush/no HBOT (C+/H-); no crush/no HBOT (C-/H-); and no crush/HBOT (C-/H+). HBOT was delivered daily for 90 minutes at three atmospheres for 10 days commencing the day of CN crush.
方法：四组Sprague-Dawley大鼠分四组：两侧海绵体神经（CN）破坏，HBOT治疗组（Crush+/HBOT+）；两侧神经破坏，非HBOT治疗组（C+/H-）；无神经破坏，无HBOT治疗组(C-/H-)；无神经破坏，HBOT治疗组(C-/H+)。CN神经破坏后即每天給予3个大气压的HBOT 90分钟×10天。
Main Outcome Measures. Ten days after CN injury, the animals underwent CN stimulation measuring the maximal intracavernosal pressure/mean arterial pressure (ICP/MAP) ratios. Corporal tissue was harvested pre-sacrifice, and immunohistochemically stained for nerve growth factor (NGF), endothelial nitric oxide synthase (eNOS), and cluster of differentiation molecule (CD31). Histologic analysis was performed for Masson's trichrome to assess the smooth muscle-collagen ratio. Terminal deoxynucleotidyl transferase Biotin-dUTP Nick End Labeling assay was used to define apoptotic indices (AIs).
主要观察指标： CN损伤10天后，大鼠接受兴奋试验，检测海绵体窦内最高压力/平均动脉压（ICP/MAP）比值。动物处死前取组织标本，并用免疫组化法标记神经生长因子（NGF）、内皮细胞一氧化氮合成酶（eNOS）、集簇分化分子（CD31）。采用Masson's trichrome分析组织平滑肌-胶原比例。末端脱氧核苷酰酶酸转移酶生物素-三磷酸去氧鸟苷缺口末端标记法评价凋亡指数(AIs)。
Results. The C+/H- group had significantly lower ICP/MAP ratios compared with C-/H- rats, (31% vs. 70%, P < 0.001). C+/H+ rats had significantly higher ICP/MAP ratio recovery compared with the C+/H- group (55% vs. 31%, P = 0.005). NGF and eNOS staining densities were higher in C+/H+ rats compared with C+/H- rats (P < 0.05 and P < 0.001, respectively). No difference was seen in CD31 expression. Staining density for MT displayed a trend toward higher smooth muscle preservation after HBOT. AIs were significantly increased by HBOT (P < 0.05).
结果：C+/H-组ICP/MAP比值显著低于C-/H-组（31% vs. 70%, P < 0.001）。C+/H+组ICP/MAP比值显著高于C+/H-组(55% vs. 31%, P = 0.005)。NGF和eNOS含量在C+/H+ 组比C+/H-组高(概率分别为P < 0.05 and P < 0.001)。CD31在各组之间无差异。MT染色密度表明接受HBOT可以更好的保护平滑肌。HBOT可使AIs显著增高(P < 0.05)。
Conclusion. HBOT following a CN injury improved EF preservation in this model, supporting the cavernosal oxygenation concept as protective mechanism for EF. The effects appear to be mediated via preservation of neurotrophic and endothelial factor expression.
结论：该模型在CN损伤后接受HBOT能保护勃起功能，该结果支持海绵体氧合可保护勃起功能这一理念。而这种保护功能可能是通过神经生长因子和内皮因子的表达等保护性因素来实现。
PMID: 18194179 [PubMed - as supplied by publisher

编译：
高压氧----海绵体神经损伤的大鼠重新勃起
介绍：海绵体氧疗在勃起组织的健康方面有重要意义。高压氧疗法（HBOT）可提高组织氧合和促进神经调节。通过研究大鼠海绵体神经损伤模型以了解HBOT在治疗勃起功能和海绵体组织方面的作用。方法：四组Sprague-Dawley大鼠分四组：两侧海绵体神经（CN）破坏，HBOT治疗组（Crush+/HBOT+）；两侧神经破坏，非HBOT治疗组（C+/H-）；无神经破坏，无HBOT治疗组(C-/H-)；无神经破坏，HBOT治疗组(C-/H+)。CN神经破坏后即每天給予3个大气压的HBOT 90分钟×10天。
主要观察指标： CN损伤10天后，大鼠接受兴奋试验，检测海绵体窦内最高压力/平均动脉压（ICP/MAP）比值。动物处死前取组织标本，并用免疫组化法标记神经生长因子（NGF）、内皮细胞一氧化氮合成酶（eNOS）、集簇分化分子（CD31）。采用Masson's trichrome分析组织平滑肌-胶原比例。末端脱氧核苷酰酶酸转移酶生物素-三磷酸去氧鸟苷缺口末端标记法评价凋亡指数(AIs)。
结果：C+/H-组ICP/MAP比值显著低于C-/H-组（31% vs. 70%, P < 0.001）。C+/H+组ICP/MAP比值显著高于C+/H-组(55% vs. 31%, P = 0.005)。NGF和eNOS含量在C+/H+ 组比C+/H-组高(概率分别为P < 0.05 and P < 0.001)。CD31在各组之间无差异。MT染色密度表明接受HBOT可以更好的保护平滑肌。HBOT可使AIs显著增高(P < 0.05)。
结论：该模型在CN损伤后接受HBOT能保护勃起功能，该结果支持海绵体氧合可保护勃起功能这一理念。而这种保护功能可能是通过神经生长因子和内皮因子的表达等保护性因素来实现。
2008-01-27 16:39
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帖者按：与1年前的文献对比一下，似乎更有道理，内皮细胞是关键，神经细胞是重点。高压氧的作用确实比较神奇怪，有临床医生经常告诉我：长期高压氧治疗能把部分老年患者白头发变黑头发。能变年轻吗？到目前为止，没有正式的文献报道。国内很多人说高压氧能治疗植物人，第一反应：吹牛。但确实有很多临床认为没什么希望的患者被救过来。希望真是这样。
氧是人体内唯一的电子最终接受体，任何其他物质都无法取代。要注意氧气是强氧化剂。我们人体是分分钟不能离开这个氧化物质的。这要与那些整天强调抗氧化的观念形成一个悖论。我们是需要氧化，还是需要还原。答案是我们需要氧化还原。不了解内情，就不要随便抗它。

ABSTRACT We hypothesized that exposure to hyperbaric oxygen (HBO2) would mobilize stem/progenitor cells from the bone marrow by a nitric oxide (.NO) dependent mechanism. The population of CD34+ cells in the peripheral circulation of humans doubled in response to a single exposure to 2.0 atmospheres absolute (ATA) O2 for 2 hours. Over a course of twenty treatments, circulating CD34+ cells increased eight-fold, although the over-all circulating white cell count was not significantly increased. The number of colony-forming cells (CFCs) increased from 16 + 2 to 26 + 3 CFCs/100,000 monocytes plated. Elevations in CFCs were entirely due to the CD34+ subpopulation, but increased cell growth only occurred in samples obtained immediately posttreatment. A high proportion of progeny cells express receptors for vascular endothelial growth
factor-2 and for stromal derived growth factor. In mice, HBO2 increased circulating stem cell factor by 50%, increased the number of circulating cells expressing stem cell antigen-1 and CD34 by 3.4-fold, and doubled the number of CFCs. Bone marrow .NO concentration increased by 1008 + 255 nM in association with HBO2. Stem cell mobilization did not occur in knock out mice lacking genes for endothelial .NO synthase. Moreover, pre-treatment of wild type mice with a nitric oxide (.NO) synthase inhibitor prevented the HBO2-induced elevation in stem cell factor and circulating stem cells. We conclude that HBO2 mobilizes stem/progenitor cells by
stimulating .NO synthesis.

[摘要：]我们设想暴露于高压氧(hyperbaric oxygen HBO2)会通过NO依赖的途径动员骨髓中干/祖细胞。单次暴露于2个绝对大气压的高压氧下2小时，外周血中CD34+细胞总数增加一倍。经过20次治疗过程，外周血中CD34＋细胞上升8倍，而外周血白细胞计数没有显著改变。每100,000单核细胞中克隆形成细胞（CFCs）数从16±2上升到26±3。上升的CFCs都是CD34+亚群，但是细胞生长加快只在治疗后立即取样的标本中出现。表达血管内皮生长因子-2 和基质来源生长因子的后裔细胞比例升高。在小鼠实验中，高压氧使外周血干细胞上升50％，外周血中表达干细胞抗原-1和CD34的细胞上升3.4倍，CFCs细胞生上升1倍。暴露高压氧后骨髓中NO浓度上升1008±255nM。内皮NO合酶基因敲除的小鼠暴露于高压氧后干细胞动员未发生。而且，野生型小鼠用NO合酶抑制剂预处理后阻止了HBO2介导的干细胞因子和外周血干细胞的增加。我们认为高压氧通过刺激NO合成动员干/祖细胞。
2008-01-30 09:02
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