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【medical-news】致炎细胞因子刺激人椎间盘细胞神经生长因子的表达

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楼主 orthoboy
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这个帖子发布于12年零148天前，其中的信息可能已发生改变或有所发展。
Proinflammatory Cytokines Stimulate the Expression of Nerve Growth Factor by Human Intervertebral Disc Cells

Abe Y, Akeda K, An HS, Aoki Y, Pichika R, Muehleman C, Kimura T, Masuda K.
Department of Orthopedic Surgery, Rush Medical College at Rush University Medical Center, Chicago IL, USA.
http://www.ncbi.nlm.nih.gov/sites/entrez

STUDY DESIGN: In vitro studies of the effects of proinflammatory cytokines on the production of nerve growth factor (NGF) by human intervertebral disc (IVD) cells.

OBJECTIVE: To determine the constitutive expression and production of NGF and the effect of cytokines on the expression of NGF by human IVD cells.

SUMMARY OF THE BACKGROUND DATA: NGF may play a role in the collateral sprouting of sensory axons, neural survival, and regulation of nociceptive sensory neurons. NGF is known to be up-regulated by proinflammatory cytokines.

METHODS: The presence of NGF protein was analyzed by immunohistochemistry using human IVD cells obtained from cadaveric human spines with no known disc disease (MRI Thompson grades 2-4). The effects of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on NGF production and mRNA expression of NGF by IVD cells were examined. The expression of NGF receptors, trkA and p75, was also assessed immunohistochemically.

RESULTS: Cadaveric anulus fibrosus (AF) and nucleus pulposus (NP) cells cultured in vitro in monolayer and in alginate beads positively stained with an anti-NGF antibody. The constitutive production of NGF protein in IVD cells was low (NP) or not detectable (AF). The expression of NGF mRNA was detectable in both cell types. IL-1beta and TNF-alpha up-regulated the NGF mRNA expression and the secretion of NGF protein into the media. TrkA was immunolocalized in AF and NP cells.

CONCLUSION: Our results demonstrate, for the first time, that human AF and NP cells constitutively express NGF protein and mRNA, and that the proinflammatory cytokines IL-1beta and TNF-alpha stimulate the production of NGF. The precise role of NGF produced by IVD cells in the generation of discogenic pain or on the metabolism of IVD cells, especially under certain physiologic conditions in which cytokines are up-regulated, needs to be clarified in future experimentation.

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Proinflammatory Cytokines Stimulate the Expression of Nerve Growth Factor by Human Intervertebral Disc Cells
致炎细胞因子刺激人椎间盘细胞神经生长因子的表达

Study Design. In vitro studies of the effects of proinflammatory cytokines on the production of nerve growth factor (NGF) by human intervertebral disc (IVD) cells.
研究设计: 对于致炎细胞因子刺激椎间盘细胞神经生长因子(NGF)作用的体外研究.

Objective. To determine the constitutive expression and production of NGF and the effect of cytokines on the expression of NGF by human IVD cells.
目的: 判断NGF的结构性表达以及产生,并人类椎间盘细胞的细胞因子对于NGF表达的作用.

Summary of the Background Data. NGF may play a role in the collateral sprouting of sensory axons, neural survival, and regulation of nociceptive sensory neurons. NGF is known to be up-regulated by proinflammatory cytokines.
背景数据概要: NGF可能在感觉神经元的侧索萌芽,神经存活,感觉神经元感受伤害的调节中发挥作用.NGF已知可以被致炎细胞因子上调.

Methods. The presence of NGF protein was analyzed by immunohistochemistry using human IVD cells obtained from cadaveric human spines with no known disc disease (MRI Thompson grades 2–4). The effects of interleukin-1_ (IL-1_) and tumor necrosis factor-_ (TNF-_) on NGF production and mRNA expression of NGF by IVD cells were examined. The expression of NGF receptors, trkA and p75NGFR, was also assessed immunohistochemically.
方法: NGF蛋白的存在使用从无已知椎间盘疾病的(MRI Thompson 2-4级)人尸体脊柱中获得的人椎间盘细胞采用免疫组化的方法分析得知.检测介素1和肿瘤坏死因子a对椎间盘细胞对于NGF产生和mRNA的表达.

Results. Cadaveric anulus fibrosus (AF) and nucleus pulposus (NP) cells cultured in vitro in monolayer and in alginate beads positively stained with an anti-NGF antibody. The constitutive production of NGF protein in IVD cells was low (NP) or not detectable (AF). The expression of NGF mRNA was detectable in both cell types. IL-1_ and TNF-_ up-regulated the NGF mRNA expression and the secretion of NGF protein into the media. TrkA was immunolocalized in AF and NP cells.
结果: 对体外在藻酸盐珠中单层培养的尸体纤维环和髓核细胞采用抗NGF抗体确切的染色.在椎间盘细胞中NGF的蛋白的结构性产生很低(髓核)或测不到(纤维环).NGF mRNA的表达在两种细胞中都有检测到.介素1和肿瘤坏死因子a上调NGF mRNA的表达和NGF蛋白的分泌.TrkA在纤维环和髓核细胞中被免疫定位.

Conclusion. Our results demonstrate, for the first time, that human AF and NP cells constitutively express NGF protein and mRNA, and that the proinflammatory cytokines IL-1_ and TNF-_ stimulate the production of NGF. The precise role of NGF produced by IVD cells in the generation of discogenic pain or on the metabolism of IVD cells, especially under certain physiologic conditions in which cytokines are up-regulated, needs to be clarified in future experimentation.
结论: 我们的研究首次表明,人类纤维环和髓核细胞可以构成性的表达NGF蛋白和mRNA, 致炎因子介素1和肿瘤坏死因a刺激NGF的产生.在椎间盘源性疼痛的产生或椎间盘细胞的代谢,特别是在某种特定的生理学条件下比如上调的细胞因子下椎间盘细胞产生的NGF的确切机制,还需要在将来的实验中进一步阐明.
2008-10-06 01:21
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楼主 orthoboy
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编译：

致炎细胞因子刺激人椎间盘细胞神经生长因子的表达

研究设计: 对于致炎细胞因子刺激椎间盘细胞神经生长因子(NGF)作用的体外研究.

目的: 判断NGF的结构性表达以及产生,并人类椎间盘细胞的细胞因子对于NGF表达的作用.

背景数据概要: NGF可能在感觉神经元的侧索萌芽,神经存活,感觉神经元感受伤害的调节中发挥作用.NGF已知可以被致炎细胞因子上调.

方法: NGF蛋白的存在使用从无已知椎间盘疾病的(MRI Thompson 2-4级)人尸体脊柱中获得的人椎间盘细胞采用免疫组化的方法分析得知.检测介素1和肿瘤坏死因子a对椎间盘细胞对于NGF产生和mRNA的表达.

结果: 对体外在藻酸盐珠中单层培养的尸体纤维环和髓核细胞采用抗NGF抗体确切的染色.在椎间盘细胞中NGF的蛋白的结构性产生很低(髓核)或测不到(纤维环).NGF mRNA的表达在两种细胞中都有检测到.介素1和肿瘤坏死因子a上调NGF mRNA的表达和NGF蛋白的分泌.TrkA在纤维环和髓核细胞中被免疫定位.

结论: 我们的研究首次表明,人类纤维环和髓核细胞可以构成性的表达NGF蛋白和mRNA, 致炎因子介素1和肿瘤坏死因a刺激NGF的产生.在椎间盘源性疼痛的产生或椎间盘细胞的代谢,特别是在某种特定的生理学条件下比如上调的细胞因子下椎间盘细胞产生的NGF的确切机制,还需要在将来的实验中进一步阐明.
2008-10-06 01:22
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